Myeloperoxidase cytochemistry using 2, 7-fluorenediamine.

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Abstract
The myeloperoxidase cytochemical reaction is well established as a valuable tool in the characterization of various types of leukocytes. This method helps in the distinction between [human] acute myeloid leukemias and acute lymphoblastic leukemias. The most common substrates used are benzidine derivatives. The known carcinogenic potential of these substrates led to widespread removal of these reagents for clinical use. Inagaki et al. (1976) reported the use of the diamine derivative of fluorene: 2,7-fluorenediamine as a good substitute of benzidine for the demonstration of myeloperoxidase in peripheral blood and bone marrow samples. Qualitatively and quantitatively both reactions were compared to assess objectively the usefulness of the new reagent.