ONLINE LIQUID-CHROMATOGRAPHIC ANALYSIS FOR DRUGS IN SERUM WITH THE TECHNICON FAST-LC SYSTEM - PERFORMANCE DATA FOR THEOPHYLLINE AND FOR 4 COMMONLY USED ANTICONVULSANTS AND THEIR METABOLITES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (7) , 871-880
Abstract
A new instrument for use in assay of therapeutic drugs in human serum by high-performance liquid chromatography, the FAST-LC system (Technicon), is described. Serum samples are aspirated directly into the unit, extracted with solvent, and the evaporated and redissolved extract is injected onto a chromatographic column. The performance of the system is illustrated by assays in serum for theophylline and 4 anticonvulsants (primidone, phenobarbital, phenytoin and carbamazepine) plus 2 of their active metabolites (phenylethylmalonamide and carbamazepine epoxide). For theophylline, final chromatograms are monitored at 270 nm, at analysis rates of 10/h. Concentration and absorbance are linearly related from 0-130 mg of theophylline/l. For the anticonvulsants, chromatograms are monitored at 200 nm, at analysis rates of 7.5/h. The 6 individual determinations are each linear beyond the therapeutic range. For both drug panels, day-to-day CV [coefficient of variability] were 4-6%. Results correlate well with those by enzyme immunoassay. A total sample volume of 150 .mu.l is required.