Interaction of Fas(Apo‐1/CD95) with proteins implicated in the ubiquitination pathway
Open Access
- 21 July 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 412 (1) , 102-106
- https://doi.org/10.1016/s0014-5793(97)00758-8
Abstract
Fas(Apo‐1/CD95), a receptor belonging to the tumor necrosis factor receptor family, induces apoptosis when triggered by Fas ligand. Upon its activation, the cytoplasmic domain of Fas binds several proteins which transmit the death signal. We used the yeast two‐hybrid screen to isolate Fas‐associated proteins. Here we report that the ubiquitin‐conjugating enzyme UBC9 binds to Fas at the interface between the death domain and the membrane‐proximal region of Fas. This interaction is also seen in vivo. UBC9 transiently expressed in HeLa cells bound to the co‐expressed cytoplasmic segment of Fas. FAF1, a Fas‐associated protein that potentiates apoptosis (Chu et al. (1996) Proc. Natl. Acad. Sci. USA 92, 11894–11898), was found to contain sequences similar to ubiquitin. These results suggest that proteins related to the ubiquitination pathway may modulate the Fas signaling pathway.Keywords
This publication has 39 references indexed in Scilit:
- FLICE, A Novel FADD-Homologous ICE/CED-3–like Protease, Is Recruited to the CD95 (Fas/APO-1) Death-Inducing Signaling ComplexCell, 1996
- Involvement of MACH, a Novel MORT1/FADD-Interacting Protease, in Fas/APO-1- and TNF Receptor–Induced Cell DeathCell, 1996
- The cell-death machineCurrent Biology, 1996
- TNF-Dependent Recruitment of the Protein Kinase RIP to the TNF Receptor-1 Signaling ComplexImmunity, 1996
- Protein Degradation or Regulation: Ub the JudgeCell, 1996
- A protein related to a proteasomal subunit binds to the intracellular domain of the p55 TNF receptor upstream to its ‘death domain’FEBS Letters, 1995
- RIP: A novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell deathCell, 1995
- FADD, a novel death domain-containing protein, interacts with the death domain of fas and initiates apoptosisCell, 1995
- Autoimmunity in Mice Bearing lprcs: A Novel Mutant GeneInternational Reviews of Immunology, 1994