FRUCTOLYSIS AND RESPIRATION OF BUFFALO SPERMATOZOA

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Abstract
The O2 uptake of buffalo semen was chiefly due to spermatozoa and there was little contribution from the seminal plasma. When rendered immotile by cold shock, buffalo spermatozoa could not utilize O2. A 2 fold increase in O2 uptake and fructolysis index of buffalo spermatozoa was seen when the pH of the suspending buffer was raised from 4.5 to 8.0. The rates for aerobic fructolysis of washed spermatozoa were comparable to those observed for whole semen. In anaerobic experiments, the fructose content decreased after the 1st and 2nd h of incubation but a consistent increase was observed during the 3rd h. Washed buffalo spermatozoa were able to utilize glucose and mannose to the same extent as fructose. Pyruvate and lactate were oxidized at a slower rate. Motility fell more rapidly than in anaerobic conditions and the spermatozoa were immotile after 3 h of incubation, indicating that buffalo spermatozoa were not able to make sufficient use of energy generated during aerobic metabolism to sustain their motility.

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