Acetylcholinesterase in the human erythron. I. Cytochemistry
- 1 August 1988
- journal article
- research article
- Published by Wiley in American Journal of Hematology
- Vol. 28 (4) , 252-259
- https://doi.org/10.1002/ajh.2830280408
Abstract
The successful demonstration and localisation of acetylcholinesterase (AChE), in cells by a cytochemical technique requires maximal expression of enzyme activity, minimal loss of AChE and precise, quantitative generation of reaction product at the actual site of the protein in vivo. These requirements are addressed in a standard technique that has been modified to avoid or optimise fixation and to exhibit enzyme activity under close-to-physiological conditions of osmolality, pH, and temperature. With these refinements and with the use of a variety of substrates and enzyme inhibitors of different specificities, true AChE was demonstrable on the membrane of erythrocytes and in the nucleus and cytoplasm of erythroblasts in bone marrow and of the constituent cells of erythroid clones in vitro. The activity in erythrocytes from umbilical cord blood was less than that in corresponding cells from the peripheral circulation of adults. AChE was observed also in human megakaryocytes and in leucocytes at all levels of differentiation, including the components of granulocyte-macrophage clones. Pseudocholinesterase was detected likewise across the spectrum of erythroid (and leucocyte) ontogeny, suggesting that these enzymes may exercise an important function in hematopoiesis.Keywords
This publication has 30 references indexed in Scilit:
- Acetylcholinesterase in the human erythron. II. Biochemical assayAmerican Journal of Hematology, 1988
- Acetylcholinesterase in red blood cellsAmerican Journal of Hematology, 1987
- Paroxysmal Nocturnal Hemoglobinuria: Erythrocyte Acetylcholinesterase Deficit Analyzed by Immunoassay and Fluorescence-Activated SortingMayo Clinic Proceedings, 1986
- Growth of erythroid colonies in agar cultures of normal human bone marrowAnnals of Hematology, 1985
- Activation of acetylcholine esterase (ACHE) as a sign for erythrocyte membrane alterationExperimental pathology, 1984
- The separation of different cell classes from lymphoid organs. V. Simple procedures for the removal of cell debris, damaged cells and erythroid cells from lymphoid cell suspensionsJournal of Immunological Methods, 1972
- Human erythrocyte membrane bound enzyme acetylcholinesteraseBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- HISTOCHEMICAL METHODS FOR SEPARATE, CONSECUTIVE AND SIMULTANEOUS DEMONSTRATION OF ACETYLCHOLINESTERASE AND NOREPINEPHRINE IN CRYOSTAT SECTIONSJournal of Histochemistry & Cytochemistry, 1967
- A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASESJournal of Histochemistry & Cytochemistry, 1964
- Über das Schicksal des Acetylcholins im BlutePflügers Archiv - European Journal of Physiology, 1928