Respiration-driven proton translocation in Escherichia coli (Short Communication)
- 15 September 1973
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 136 (1) , 217-220
- https://doi.org/10.1042/bj1360217
Abstract
Measurements were made of the stoicheiometry of respiration-driven proton translocation coupled to the oxidation of NAD(P)-linked or flavin-linked substrates in intact cells of Escherichia coli. Observed stoicheiometries (→H+/O quotient; Mitchell, 1966) were approx. 4 with l-malate as substrate and approx. 2 for succinate, d-lactate and glycerol oxidation. It is concluded that the potential number of equivalent energy-conservation sites associated with the respiratory chain is 2 in aerobically grown cells of E. coli harvested during the exponential phase of growth.Keywords
This publication has 27 references indexed in Scilit:
- Different Mechanisms of Energy Coupling for the Active Transport of Proline and Glutamine in Escherichia coliProceedings of the National Academy of Sciences, 1973
- Electron‐Transport Chains of Escherichia coliEuropean Journal of Biochemistry, 1973
- Oxidative phosphorylation in intact bacteriaArchiv für Mikrobiologie, 1973
- Proton translocation coupled to quinone reduction by reduced nicotinamide–adenine dinucleotide in rat liver and ox heart mitochondriaBiochemical Journal, 1972
- Coupling of Energy to Active Transport of Amino Acids in Escherichia coliProceedings of the National Academy of Sciences, 1972
- The Effect of Growth Conditions on Respiratory Activity and Growth Efficiency in Facultative Anaerobes Grown in Chemostat CultureJournal of General Microbiology, 1971
- Bacterial Cytochromes: I. Structural AspectsAnnual Review of Biochemistry, 1970
- Studies of the efficiency of oxidative phosphorylation in intact Escherichia coli BBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1970
- Energy coupling in the transport of beta-galactosides by Escherichia coli: effect of proton conductors.1969
- THE LACTIC DEHYDROGENASES OF E. COLI*Annals of the New York Academy of Sciences, 1965