ARF1‐regulated phospholipase D in human neutrophils is enhanced by PMA and MgATP
- 26 September 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 352 (2) , 113-117
- https://doi.org/10.1016/0014-5793(94)00930-9
Abstract
Human neutrophil PLD activity stimulated with GTP‐γ‐S was reconstituted with recombinant ARF1 in cytosol‐depleted cells. PMA‐pretreatment of intact cells greatly enhanced the subsequent reconstitution of the ARF1‐regulated PLD activity. This enhancement was only observed provided that the intact cells were pretreated with PMA, suggesting the stable recruitment of a cytosolic component, presumably protein kinase C, to the membranes. rARF1‐reconstituted PLD activity was not dependent on MGATP, but could be considerably enhanced by MgATP. Maximal effects of MgATP were seen at 1 mM. This enhancement by MgATP could not be attributed to protein kinase C. Neomycin was found to inhibit ARF1‐regulated PLD activity suggesting the requirement for polyphosphoinositides. We conclude: (i) that many of the observed effects of PMA may be dependent on the presence of the small GTP‐binding protein, ARF, and (ii) polyphosphoinositides are required for ARF1‐stimulated PLD activity.Keywords
This publication has 34 references indexed in Scilit:
- A novel phosphoinositide 3 kinase activity in myeloid-derived cells is activated by G protein βγ subunitsCell, 1994
- Phospholipase D activation in fibroblast membranes by the α and β isoforms of protein kinase CFEBS Letters, 1994
- Phospholipase D: a Downstream Effector of ARF in GranulocytesScience, 1994
- Rat brain cytosol contains a factor which reconstitutes guanine‐nucleotide‐binding‐protein‐regulated phospholipase‐D activation in HL60 cells previously permeabilized with streptolysin OEuropean Journal of Biochemistry, 1993
- Protein kinase C isotypes and signal-transduction in human neutrophils: Selective substrate specificity of calcium-dependent β-PKC and novel calcium-independent nPKCBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1993
- Phospholipase D and cell signalingCurrent Opinion in Immunology, 1993
- C-protein-regulated phospholipases C, D and A2-mediated signalling in neutrophilsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1992
- Endothelin stimulates phosphatidic acid formation in cultured rat mesangial cells: Role of a protein kinase C‐regulated phospholipase DJournal of Cellular Physiology, 1992
- Granulocyte phospholipase D is activated by a guanine nucleotide dependent protein factorBiochemical and Biophysical Research Communications, 1991
- Two G-proteins act in series to control stimulus-secretion coupling in mast cells: use of neomycin to distinguish between G-proteins controlling polyphosphoinositide phosphodiesterase and exocytosis.The Journal of cell biology, 1987