Analysis of Nod-Factor-Induced Calcium Signaling in Root Hairs of Symbiotically Defective Mutants of Lotus japonicus
Open Access
- 1 August 2006
- journal article
- research article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 19 (8) , 914-923
- https://doi.org/10.1094/mpmi-19-0914
Abstract
Nodulation (Nod)-factor signaling molecules are essential for rhizobia to initiate the nitrogen-fixing symbiotic interaction with legumes. Using a dual dye ratiometric calcium imaging technique, we have shown that 10 nM Nod factor added to roots of Lotus japonicus seedlings induces an intra-cellular calcium increase (calcium flux) that precedes oscillations in intracellular calcium (calcium spiking). The calcium flux was not observed with 1 or 0.1 nM Nod factor, which did induce calcium spiking. The calcium flux was variable in timing of initiation and duration and was observed in approximately half of the root hairs examined. Representatives from 11 complementation groups of symbiotically defective mutants were analyzed for the calcium flux. Mutants from four groups (sym6, ccamk, sym35, and nin) which retained calcium spiking all showed a normal calcium flux. Two classes of mutants (nfr1 and nfr5) lacked both calcium influx and calcium spiking, whereas five classes of mutants (symRK, castor, pollux, nup1...Keywords
This publication has 58 references indexed in Scilit:
- Regulation of Plant Symbiosis Receptor Kinase through Serine and Threonine PhosphorylationJournal of Biological Chemistry, 2005
- Plastid proteins crucial for symbiotic fungal and bacterial entry into plant rootsNature, 2004
- A Putative Ca 2+ and Calmodulin-Dependent Protein Kinase Required for Bacterial and Fungal SymbiosesScience, 2004
- Medicago truncatula DMI1 Required for Bacterial and Fungal Symbioses in LegumesScience, 2004
- LysM Domain Receptor Kinases Regulating Rhizobial Nod Factor-Induced InfectionScience, 2003
- A receptor kinase gene of the LysM type is involved in legumeperception of rhizobial signalsNature, 2003
- Plant recognition of symbiotic bacteria requires two LysM receptor-like kinasesNature, 2003
- Registration of GIFU B‐129‐S9 Lotus japonicus GermplasmCrop Science, 1996
- Rhizobium meliloti lipooligosaccharide nodulation factors: different structural requirements for bacterial entry into target root hair cells and induction of plant symbiotic developmental responses.Plant Cell, 1994
- Rhizobium meliloti Nod factors elicit cell‐specific transcription of the ENOD12 gene in transgenic alfalfaThe Plant Journal, 1994