Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell-to-cell movement
Open Access
- 1 March 2000
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 19 (5) , 913-920
- https://doi.org/10.1093/emboj/19.5.913
Abstract
Virus‐encoded movement protein (MP) mediates cell‐to‐cell spread of tobacco mosaic virus (TMV) through plant intercellular connections, the plasmodesmata. The molecular pathway by which TMV MP interacts with the host cell is largely unknown. To understand this process better, a cell wall‐associated protein that specifically binds the viral MP was purified from tobacco leaf cell walls and identified as pectin methylesterase (PME). In addition to TMV MP, PME is recognized by MPs of turnip vein clearing virus (TVCV) and cauliflower mosaic virus (CaMV). The use of amino acid deletion mutants of TMV MP showed that its domain was necessary and sufficient for association with PME. Deletion of the PME‐binding region resulted in inactivation of TMV cell‐to‐cell movement.Keywords
This publication has 45 references indexed in Scilit:
- Inhibition of plant viral systemic infection by non‐toxic concentrations of cadmiumThe Plant Journal, 1998
- Selective Trafficking of KNOTTED1 Homeodomain Protein and Its mRNA Through PlasmodesmataScience, 1995
- Tobacco mosaic virus movement protein associates with the cytoskeleton in tobacco cells.Plant Cell, 1995
- Completion of a cDNA sequence from a tobamovirus pathogenic to crucifersGene, 1995
- Long-distance movement of viruses in plantsTrends in Microbiology, 1993
- Phosphorylation of tobacco mosaic virus cell-to-cell movement protein by a developmentally regulated plant cell wall-associated protein kinase.Genes & Development, 1993
- Developmental changes in plasmodesmata in transgenic tobacco expressing the movement protein of tobacco mosaic virusProtoplasma, 1992
- Substructure of freeze-substituted plasmodesmataProtoplasma, 1992
- A novel genetic system to detect protein–protein interactionsNature, 1989
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970