The Action of alpha-Chymotrypsin on Tyrosine Ethyl Ester.

Abstract
The splitting of tyrosine ethyl ester by [alpha]-chymotrypsin was studied by means of continuous titration in a pH-stat. The reaction was followed to about 99% hydrolysis, and to explain the obtained results it is necessary to assume the simultaneous or consecutive uptake of three molecules of substrate before reaction takes place. This supports the view that transpeptidation is the first step in the splitting of this substrate.