Separation and sugar component analysis of the oligosaccharides in the surface glycoproteins of Newcastle Disease Virus

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Abstract
The precursor glycoproteins HN0 and F0 in the surface spikes of Newcastle Disease Virus strain Ulster as produced by MDBK cells, were found to contain 10.4 and 11.9 weight per cent, respectively, of the sugars typical forN-glycosidically linked glycoprotein glycans. A molar ratio ofD-mannose:D-galactose:L-fucose:N-acetyl-D-glucosamine approaching 1.0:1.1:0.5:1.0 was found for HN0, and of 1.0:0.7:0.3:0.6 for F0. By a sequence of degradation (with pronase, with endo-β-N-acetylglucosaminidase H [endo H], and by hydrazinolysis) and separation procedures (Concanavalin A-affinity and Biogel P-4 chromatography), the radiolabelled carbohydrate moieties of NDV HN0 and F0 (as oligosaccharitols) were separated into (at least) ten and eight fractions, respectively. Separatein vivo labelling with tritiated derivatives of the four sugars showed that both glycoproteins contain oligosaccharides of the oligomannosidic (“high mannose”), of theN-acetyllactosaminic (“complex”), as well as of the “mixed” type. The majority of the oligosaccharides in F0, but not of those in HN0, was found to be endo H-sensitive.