Components of neurite outgrowth that determine neuronal cytoarchitecture: Influence of calcium and the growth substrate

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Abstract
The present study examined the cellular mechanisms underlying the generation of neuroarchitecture. Identified Helisoma neurons in isolated cell culture normally require factors present in brain‐conditioned medium (CM) in order to display the different components of neurite outgrowth (sprouting, elongation, branching, and growth cone motility), which ultimately determine their overall architecture. We report here that cell calcium and cell‐substrate interactions can play quite specific roles in the regulation of these different components of neuronal outgrowth. CM‐induced neurite outgrowth was inhibited by calcium ionophore A23187. In the absence of CM the calcium channel blocker La3+ (10μM) reduced intracellular calcium levels and induced neurite sprouting and elongation; growth cone motility and branching were greatly reduced in the La3+ ‐induced neurites. Neurons plated into an environment containing La3+ and a fibronectin substrate exhibited all of the components of neuronal outgrowth normally seen in response to CM. Fibronectin alone had little outgrowthpromoting activity. Neurite elongation rates and branching were increased by exposure to La3+ in neurons on either a CM or fibronectin substrate. The neurons growing on CM or fibronectin whose outgrowth was accelerated by La3+ elaborated neuritic arbors that differed from those of neurons grown in resposne to CM; differences were seen in neurite length, area of outgrowth, branching frequency, and varicosity numbers. Taken together, these results indicate that (1) calcium and the growth substrate can exert specific effects on neurite sprouting, elongation, growth cone motility, and branching; (2) appropriate levels of activation of these two sytems can elicit neurite outgrowth that closely resembles that induced by endogenous growth factors; (3) both the differential expression of the separate components of outgrowth and the kinetics of outgrowth determine a neuron' morphology.