An Improved Sum-Of-Normals Technique For Cell Cycle Distribution Analysis of Flow Cytometric Dna Histograms

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Abstract
An improved method for determining the fraction of cells in the G1, S and G2 + M phases of the life cycle, from DNA distributions of S phase rich cultures [Chinese hamster ovary, CHO cell], is presented. Picolinic acid synchronized cell cultures were used to demonstrate the usefulness of the technique for the cell cycle distribution analysis. The algorithm presented quantifies DNA histograms from FCM [flow cytometry method] analyses using an extension of the previously reported sum of discrete normal curves technique. It alleviates problems encountered with earlier methods by extending the contribution of the S phase cells to the distribution under the G1 and G2 + M peaks; at the same time it allows more of the descriptive parameters to be determined from the individual histograms. The fractions of cells in the G1, S, and G2 + M phases of the life cycle obtained by this method compare favorably with autoradiographic analyses and other analytical results.