5‘-RNA Self-Capping from Guanosine Diphosphate
- 1 June 1997
- journal article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 36 (22) , 6557-6563
- https://doi.org/10.1021/bi970475b
Abstract
A selected RNA (isolate 6) efficiently catalyzes a self-capping reaction with free GDP, yielding the same 5‘-capped structure as is formed by protein GTP:RNA guanylyltransferase. This unexplored RNA-catalyzed reaction type involving nucleophilic attack on phosphate by phosphate adds to the variety of possible postsynthetic RNA-catalyzed RNA modifications. The selected RNA requires only Ca2+ for activation and has a broad active pH range of 4.5−9.0. The RNA also has a 5‘-pyrophosphatase activity.Keywords
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