HIV-1 A-rich RNA loop mimics the tRNA anticodon structure
- 1 December 1998
- journal article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 5 (12) , 1033-1036
- https://doi.org/10.1038/4141
Abstract
Interaction of HIV-1 genomic RNA and human tRNA(Lys)3 initiates viral reverse transcription. An adenosine-rich (A-rich) loop in HIV RNA mediates complex formation between tRNA and viral RNA. We have determined the structure of an A-rich loop oligonucleotide using nuclear magnetic resonance spectroscopy. The loop structure is stabilized by a noncanonical G-A pair and a U-turn motif, which leads to stacking of the conserved adenosines. The structure has similarity to the tRNA anticodon structure, and suggests possible mechanisms for its role in initiation of reverse transcription.Keywords
This publication has 31 references indexed in Scilit:
- HIV-1 Regulatory/Accessory Genes: Keys to Unraveling Viral and Host Cell BiologyScience, 1998
- Mutational analysis of the tRNA3Lys/HIV-1 RNA (primer/template) complexNucleic Acids Research, 1998
- Structure of the HIV-1 Nucleocapsid Protein Bound to the SL3 Ψ-RNA Recognition ElementScience, 1998
- Interaction of Retroviral Reverse Transcriptase with Template–Primer Duplexes during ReplicationProgress in Nucleic Acid Research and Molecular Biology, 1997
- α Helix-RNA Major Groove Recognition in an HIV-1 Rev Peptide-RRE RNA ComplexScience, 1996
- Initiation of Reverse Transcripion of HIV-1: Secondary Structure of the HIV-1 RNA/tRNA|rlmbopopnbop|Lys|clobop|3 (Template/Primer) ComplexJournal of Molecular Biology, 1995
- Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA.Proceedings of the National Academy of Sciences, 1993
- Role of RNA structure in arginine recognition of TAR RNA.Proceedings of the National Academy of Sciences, 1993
- Crystal Structure at 3.5 Å Resolution of HIV-1 Reverse Transcriptase Complexed with an InhibitorScience, 1992
- π Turn is a conformational pattern in RNA loops and bendsNature, 1976