Separation of Germ Cells from Somatic Cells in Mouse Testis by Affinity for a Lectin, Peanut Agglutinin

Publisher Website
Google Scholar
Abstract
A new method for the separation of germ cells from somatic cells in the mouse testis was accomplished by making use of the differences in cell surface affinity for a lectin, peanut agglutinin (PNA). The separation procedure was based on the specific presence of PNA receptor on testicular germ cells and its absence on somatic cells, such as Leydig, Sertoli and peritubular cells. Of cells in PNA receptor-positive (PNA+) fractions > 99% were identified as germ cells by immunoperoxidase reaction with specific antiserum to mouse testicular germ cells. Leydig cells were enriched in PNA receptor-negative (PNA-) fractions, i.e., 65% of cells in these fractions were cytochemically stained for 3 .beta.-hydroxysteroid dehydrogenase (3.beta.-HSD) activity.