Metabolism of cartilage proteins in cultured tissue sections

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Abstract
The biosynthesis and turnover of cartilage proteins was studied in organ cultures of bovine tracheal cartilage sections. In cultures labeled with [3H]leucine, more than 99% of the labeled macromolecules were retained in the sections. About 50% of the [3H]leucine-labeled protein was extracted with 4M-guanidinium chloride. The incorporation of [3H]leucine into protein extractable with guanidinium chloride was linear with time, after an initial delay of 20-25 min. The 148 kDa [dalton] and 36 kDa cartilage proteins were major labeled components in this extract. The elimination of the proteins was studied by using a pulse-chase protocol. The 148 kDa protein had a very slow turnover, similar to that of proteoglycans, while the 36 kDa protein was eliminated more rapidly.