Abstract
Various attempts to sensitize mice to Ni salts are described. The antigen was applied once or repeatedly by the epicutaneous or intracutaneous route and by combinations of both. The procedures included prior irritation of the skin, treatment with Freund''s complete adjuvant, cyclophosphamide or tetramisole and prior sensitization to picryl chloride. Contact allergy was achieved only by repeated epicutaneous application of a strong Ni solution over a 3-wk period. The resulting dermatitis was demonstrated by a weak but significant wet weight increase of inflamed skin.

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