Trimethoprim-Sensitivity Testing and Thymineless Mutants

Abstract
Various laboratory media were examined for use in trimethoprim-sensitivity testing. The most suitable were Wellcotest Sensitivity Test Agar and Wellcome Nutrient Agar, because these were the least antagonistic to the action of trimethoprim. Thymine as well as thymidine were found to antagonise trimethoprim activity against wild-type bacteria, and both these compounds are absent from the above media.

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