Column Chromatography of Allergens and Assessment of their Activities by the Paper Disc Radioallergosorbent Method

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Abstract
Various birch-pollen and dog-epithelium allergen extracts were chromatographed using Sephadex G-75 and Sephadex G-200 columns. The chromatographic tractions were then tested by an in vitro paper disc radioallergosorbent test, using sera from allergen-sensitive patients. Calibrated columns were used to approximate molecular weights of some birch-pollen and dog-epithelium allergenic active components.