Optimization of Thin Layer Chromatography for Toxicological Screening: Applicability of Shorter Development Distances

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Abstract
Over the years, numerous TLC systems have been developed, each of them having their special advantages. Yet little or no attention has been paid to the time-factor needed to obtain sufficiently reliable Rf-values, combined with satisfactory resolution. As speed remains an important factor in toxicology, the applicability of shorter development distances have been investigated, resulting in a considerable gain in time as the development times increase exponentially with developing distance. In this paper, the effects of shorter development distances on resolution, spot size, reproducibility of Rf-values, and sensitivity of detection are described.

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