Electrophoresis And Electrofocusing of Venom of Loxosceles Reclusa Gertsch & Mulaik (Araneida: Scytodidae)1

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Abstract
Venom from Loxosceles reclusa was extracted by electrical stimulation and resolved by gel electrophoresis and gel electrofocusing techniques. Eight venom proteins were consistently resolved from male and female L. reclusa when electrophoretically analyzed. Qualitative and quantitative patterns were noted when the sexes were contrasted. Gel electrofocusing revealed 16–22 venom protein components in the females and 11–20 in the males. Gel electrofocusing was superior to electrophoresis in separation of L. reclusa venom proteins and thus provides an additional parameter in the enzymatic characterizationof the venom components.