Fine structure of iron ion in deoxyhemoglobin and deoxymyoglobin

Abstract
To study the mechanism of oxygen binding of hemoglobin and myoglobin the electronic state of the central Fe2+ion was determined by means of magnetic susceptibility measurements. The paramagnetic saturation under high magnetic field of a superconducting magnet and the torque of a single crystal of hemoglobin were also studied. It was found that the fine structure of the lowest ground-state energy level (5B2) of ferrous ion in hemoglobin and myoglobin could be well represented by the spin Hamiltonian . The value of determined from the susceptibility measurements and from the field dependence of magnetization was 5 cm-1. From the torque measurements, the values of and were also estimated to be 5.3 and 0.9 cm-1, respectively.

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