OVARIAN ASCORBIC ACID AS AN ENDOGENOUS AND EXOGENOUS ASSAY FOR CYCLIC PROESTROUS LH RELEASE1

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Abstract
Cyclic proestrous LH release was investigated by endogenous and exogenous assay in rats exposed to light from 5:00 A.M. to 7:00 P.M. daily. Pituitaries were removed from proestrous rats at 10:00–11:00 A.M. and 4:00–5:00 P.M., injected into immature, primed recipient rats and tested for ovarian ascorbic acid depleting ability (exogenous assay). “Morning” pituitaries were significantly more potent than “afternoon” pituitaries, confirming the finding of drug and hypophysectomy studies that LH is released from the pituitary between 2:00 and 3:00 P.M. Ovaries removed from the proestrous donor rats showed no consistent ascorbic acid depletion on the afternoon of proestrus (endogenous assay). There was, however, a cyclic variation in ovarian ascorbic acid, the highest values occurring in metestrus, the lowest in proestrus.

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