PURIFICATION OF STAPHYLOCOCCAL ALPHA-HEMOLYSIN

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Abstract
Madoff, MortonA. (New England Center Hospital, Boston, Mass.)and Louis Weinstein. Purification of staphylococcal alpha-hemolysin. J. Bacteriol.83:914–918. 1962. — Staphylococcal alpha-toxin has been purified by metal-ion precipitation, column chromatography, and continuous-flow paper electrophoresis. The resultant preparation is highly unstable. A single line is obtained on agar-gel diffusion analysis against staphylococcal antiserum. Two bands, both associated with hemolytic activity and migrating cathodally at pH 8.4, are obtained on starch-gel electrophoresis. Levels of lytic, dermonecrotic, and lethal toxin activity maintain a constant relationship in the crude and purified materials, suggesting the unity of these effects.