Isolation and characterization of Aphanocladium album chitinase-overproducing mutants

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Abstract
Summary: A wild-type strain of the fungus Aphanocladium album was mutagenized by UV irradiation in order to obtain chitinase-overproducing mutants. Mutants were screened on agar medium containing colloidal chitin and selected for their ability to produce large clearing zones around the colonies. Two mutant strains, designated E3 and E12, showed respectively a 26- and a 2·5-fold increase in maximal extracellular chitinase activity, determined in liquid medium with crystalline chitin as sole carbon source, compared to the wild-type strain. This is believed to be the first report on the induction of stable chitinase-overproducing mutants in a filamentous fungus. Regulation of enzyme activity was investigated in mutant E3 and the wild-type strain.

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