Transformation of normal rat kidney (NRK) cells by an infectious retrovirus carrying a synthetic rat type alpha transforming growth factor gene.

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Abstract
We synthesized a gene for rat type .alpha. transforming growth factor (TGF-.alpha.) consisting of the leader sequence and the sequence coding for the mature 50-amino acid peptide without the C-terminal processed region. This gene was inserted into the retrovirus vector pSW272, which is derived from spleen necrosis virus, to obtain an infectious recombinant virus carrying the rat TGF-.alpha. gene. This recombinant virus can infect normal rat kidney (NRK) cells and allow these cells to grow in soft agar in the presence of TGF-.beta.. Transformed cells isolated from colonies grown in soft agar contain an integrated form of the recombinant virus and secrete biologically active TGF-.alpha. into their medium. These results show that a biologically active TGF-.alpha. can be produced by a gene synthesized from only part of the coding region and that the infectious retrovirus carrying the TGF-.alpha. gene (SW355) can function as a transforming agent in NRK cells in the presence of TGF-.beta.