REVERSAL OF AZASERINE BY PHENYLALANINE

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Abstract
Azaserine reacts irreversibly with cells in phosphate buffer and brings about characteristic changes when these cells are subsequently incubated in growth medium. The antagonism of L-azaserine by phenylalanine has been shown to be due to a blocking of this binding or uptake of azaserine by the cells. When cells treated first with azaserine are used, phenylalanine has no effect on its action. Phenylalanine will reverse if it is present during azaserine treatment and has only a partial effect if the cells are grown in phenylalanine before treatment. Some effects are described of azaserine on Escherichia coli strain ML35, a mutant which forms [beta]-galactosidase constitutively and lacks a permease for 0-galactosides. Some of the effects noted are probably secondary effects due to induction of phage production in this presumably lysogenic strain.

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