Further Investigations Relative to Studies with I131-Labeled Proteins, Insulin in Particular.

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Abstract
An appreciable portion of inorganic I131 was found to be precipitated by trichloracetic acid (TCA) in the presence of red blood cells. This was not the case with serum or other tissues studied. In evaluating the degradation of I131 labeled proteins by measuring the TCA precipitability of the blood radioactivity, it should, therefore, be appreciated that the degradation product, inorganic I131, may show the phenomenon of "re-entry," and be precipitated by TCA. Findings, somewhat similar to those with inorganic I131 were also obtained with I131 labeled diiodotyrosine.

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