ACTIVATION AND PROLIFERATION SIGNALS IN MOUSE B-CELLS .3. INTACT (IGG) ANTI-IMMUNOGLOBULIN ANTIBODIES ACTIVATE B-CELLS BUT INHIBIT INDUCTION OF DNA-SYNTHESIS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 53  (4) , 693-701
Abstract
Intact (IgG) rabbit anti-Ig antibodies are generally not mitogenic for mouse B cells but inhibit proliferation induced by either F(ab'')2 anti-Ig or by lipopolysaccharide. It is shown here, however, that IgG anti-Ig activates mouse B cells, since it causes B cells to depolarize, enlarge and express increased levels of Ia antigens. In the continuing presence of IgG anti-Ig, B cells do not synthesize DNA. However, if cells cultured with IgG antibody are then washed, they start to proliferate earlier in response to F(ab'')2 anti-Ig, i.e., they have become primed. Therefore IgG anti-Ig is an example of a step-1 activator for mouse B cells, which drives resting B cells out of Go, but actively prevents the cells from progressing into S. The latter effect appears to result from cross-linking of surface Ig and Fc receptors on B cells, although the mechanism is, as yet, unknown.

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