Abstract
The morphology of the X chromosome in the salivary glands of old larvae is described in detail. A study is then made of a number of X-4 translocations and other aberrations. Knowing genetically between what 2 genes a given break has occurred and cytologically just where the X is broken, it is possible to give morphological limits to gene loci. In this manner the approximate positions of 21 gene loci scattered well along the X have been given. In addition to this new type of chromosome map, the following points are stressed: (a) somatic synapsis occurs in old larvae, (b) the pattern of lines and bands on the various segments of the X are constant, (c) neither the inert region of the X nor the Y chromosome show the banded form in the salivary gland.

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