Transforming growth factor‐β1 inhibits expression of selenoprotein P in cultured human liver cells
Open Access
- 22 October 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 460 (1) , 23-26
- https://doi.org/10.1016/s0014-5793(99)01298-3
Abstract
The effect of cytokines on the expression of selenoprotein P (SeP) in the human liver cell line HepG2 was investigated. Treatment with interleukin‐1β, interferon‐γ, and tumor necrosis factor‐α had no effect on SeP levels in culture media or on SeP mRNA expression. Conversely, Western analysis revealed a dose‐dependent reduction of SeP content in culture medium after treatment with transforming growth factor (TGF)‐β1 with an IC50 of 31 pM. Treatment with 100 pM TGF‐β1 for 48 h led to a decrease to 21±9% of controls. RT‐PCR analysis of SeP mRNA expression demonstrated an inhibition of SeP transcription to 40±2% of control levels after 24 h. The expression of a luciferase reporter construct under control of the human SeP promoter was downregulated by TGF‐β1 treatment in a dose‐dependent fashion indicating a transcriptional regulation of the SeP gene by TGF‐β1.Keywords
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