Enhancement of transformation rates in higher plants by low-dose irradiation: Are DNA repair systems involved in the incorporation of exogenous DNA into the plant genome?
- 1 February 1989
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 12 (2) , 189-199
- https://doi.org/10.1007/bf00020504
Abstract
Irradiation (X-ray; 5–15 Gy) of protoplasts treated with plasmid-DNA and PEG yielded higher transformation rates in comparison to non-irradiated protoplasts transformed by the same method. This could be demonstrated for four plant species. The irradiation doses used did not affect the total number of colonies regenerated without selection pressure, but resulted in 3–6-fold enhancement of hygromycin- or kanamycin-resistant colonies. Plant regeneration frequencies of transformed colonies derived from irradiated and non-irradiated protoplasts were similar in tobacco as well as in Petunia. Higher integration rates of foreign DNA as a consequence of an increased recombination machinery in irradiated cells may be responsible for the enhancement of the number of stably transformed colonies.Keywords
This publication has 34 references indexed in Scilit:
- Effect of X‐ray Irradiation on Maize Inbred Line B73 Tissue Cultures and Regenerated PlantsCrop Science, 1988
- DNA repair in pollen. A reviewMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1987
- Chromosome and DNA damage and their repair in higher plants irradiated with short-wave ultraviolet lightMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1987
- Formation, detection and repair of AP sitesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1987
- Inducible responses to dna damage in bacteria and mammalian cellsEnvironmental and Molecular Mutagenesis, 1987
- Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusionMolecular Genetics and Genomics, 1983
- DNA repair synthesis in plant protoplasts is aphidicolin‐resistantFEBS Letters, 1982
- Regeneration von haploiden und diploiden pflanzen aus protoplasten von Petunia hybrida L.Zeitschrift für Pflanzenphysiologie, 1975
- Repair of DNA strand breaks after gamma-irradiation of protoplasts isolated from cultured wild carrot cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1975
- The Implication of Repair Processes in the Mechanism of DNA Integration by Lymphoma CellsInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1969