MICROANALYSIS OF MITOCHONDRIAL GRANULES. MICROANALYSIS OF MITOCHONDRIAL GRANULES IN BONE CELLS INCIDENT TO EXPERIMENTAL TOOTH MOVEMENT

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Abstract
Molars of adult Wistar rats were moved experimentally by orthodontic elastic for 4 days. Energy dispersive X-ray microanalysis of mitochondrial granules in bone cells incidental to experimental tooth movement was then performed. In the unstained specimens treated with OsO4-glutaraldehyde, microanalysis can be applied to a portion of the elements. A condition in which Ca may bind strongly with protein in mitochondria is shown. At mineralizing sites in the experimental groups, Ca-K, P-K and Os-M X-ray lines were detected in mitochondrial granules in morphologically active osteoblasts and young osteocytes, but the P-K and Os-M peaks could not be separated from their combined peak. At absorbing mineral sites Ca-K was found markedly in the ruffled borders and vacuoles of osteoclasts. The Ca-K X-ray line was also detected in mitochondrial granules of osteoclasts. In the control groups mitochondria of resting osteoblasts and osteocytes had few granules, and the detection of the Ca-K X-ray line from these mitochondria was very difficult. The Ca-K X-ray line was detected in the mitochondria close to the ruffled border of the osteoclasts, but it was not detected in the mitochondria located on the vascular side of the cells.