ELECTROPHORESIS OF AUTOPROTHROMBIN AND BIOTHROMBIN

Abstract
Concentrates of biothrombin and autoprothrombin were prepared from purified prothrombin and subjected to electrophoresis. The mobility of biothrombin is less than that of prothrombin. The mobility of autoprothrombin is less than that of biothrombin. The biothrombin and autoprothrombin preparations examined were not electro-phoretically homogeneous. Prothrombin dissociates during electrophoresis at high voltage gradients. Since this does not occur with biothrombin and autoprothrombin, it seems likely that electrophor-etically homogeneous derivatives of prothrombin can be obtained with further purification work.
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