Inhibitory effect of caffeine on 5‐azacytidine‐induced digital malformations in the rat

Abstract
The effect of caffeine on 5‐azacytidine (5‐AC)‐induced digital malformations in rat fetuses was investigated. Caffeine suppressed all types of digital defects in the fore‐ and hindlimbs except for syndactyly induced by 1.0 mg/kg of 5‐AC; it was still effective when administered 24 hours after 5‐AC treatment. However, fetal mortality increased as the frequency of malformations decreased. While the malformation results support the view that caffeine inhibits the processes leading to malformation expression, the relation between its suppressive effect on malformations and its enhancing effect on fetal mortality is unclear.

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