The effects of various gastrins on intracellular free Ca2+in isolated pig parietal cells

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Abstract
Gastrin 17 (G17) is a potent stimulant of gastric acid secretion in vivo. In this study, the effects of G17 and some related on intracellular free Ca2+ in isolated pig parietal cells were studied. Both G17 and the synthetic peptide pentagastrin increased intracellular free Ca2+ in a dose-dependent manner over the concentration range 10-6 to 10-6 M, suggesting a specific action. The EC50 values were 3 .times. 10-8 M for G17 and 8 .times. 10-8 M for pentagastrin. The N-terminal tridecapeptide of G17 [(1-13)G17] did not have any effect on intracellular free Ca2+, nor was it able to inhibit the action of G17. A glycine-extended gastrin [(5-17)G17-Gly)] elicited a small but significant increase in intracellular free Ca2+ although only at 10-6 M. This increase was approximately 20% of that obtained with a similar concentration of G17. Sequential incubations with (5-17)G17 and G17 showed that both peptides increased the intracellular free Ca2+ through the same mechanisms.

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