Reduced Ability of Neutrophils to Produce Active Oxygen Species in Streptozotocin-Induced Diabetic Rats
- 1 January 1992
- journal article
- research article
- Published by Georg Thieme Verlag KG in Experimental and Clinical Endocrinology & Diabetes
- Vol. 99 (01) , 31-33
- https://doi.org/10.1055/s-0029-1211128
Abstract
We investigated a possible alteration in the ability of neutrophils to produce active oxygen species in streptozotocin (STZ)-induced diabetic rats. The production of Superoxide of the neutrophils was assessed by luminol-dependent chemiluminescence (LDCL) after the stimulation by opsonized zymosan. Four days after STZ (60 mg/kg, ip) injection, blood glucose level increased by 399 ± 9 mg/dl and the LDCL activity was significantly reduced in diabetic rats (control group: 5.12±1.53 KC/min2/106 cells, STZ group: 1.10±0.07 KC/ min2/106 cells, p2/106 cells) was almost at the same level as that of diabetic rats at Day 4. Subcutaneous injection of insulin for 10 consecutive days increased the LDCL activity of diabetic rats in a dose-dependent manner (vehicle: 0.82±0.27 KC/ min2/106 cells, 0.04 U/day: 1.61 ±0.09 KC/min2/106 cells, 0.40 U/day: 1.99±0.47 KC/min2/106 cells, 4.00 U/day: 3.33 ±0.43 KC/min2/106 cells). The data obtained herein indicate that an increased susceptibility to bacterial infection in diabetic rats results from impaired neutrophil function to produce active oxygen species.Keywords
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