The occurrence of a 70‐kDa serine protease gene in typical and atypical strains of Aeromonas salmonicida
- 1 November 1992
- journal article
- Published by Wiley in Journal of Fish Diseases
- Vol. 15 (6) , 529-535
- https://doi.org/10.1111/j.1365-2761.1992.tb00685.x
Abstract
The typical strains of Aeromonas salmonicida examined secreted a PMSF‐sensitive proteolytic activity, and with few exceptions, gave a positive colony hybridization with a 70‐kDa scrinc protease gene probe. By contrast, atypical strains produced a spectrum of responses with various combinations of (1) secreted/did not secrete PMSF‐inhibited protease, (2) colonies hybridized/did not hybridize with the gene probe, and (3) produced protease not inhibited by PMSF. It is suggested that certain strains might be reassigned as typical or atypical on the basis of the presence or absence of the protease gene.Keywords
This publication has 7 references indexed in Scilit:
- An appraisal of the extracellular toxins ofAeromonas salmonicidassp.salmonicidaJournal of Fish Diseases, 1991
- Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinasesProtein Engineering, Design and Selection, 1991
- The molecular evolution of genes and proteins: a tale of two serinesNature, 1988
- Identification of major common extracellular proteins secreted by Aeromonas salmonicida strains isolated from diseased fishApplied and Environmental Microbiology, 1987
- A study of the pathological effect of isolated Aeromonas salmonidda extracellular protease on Atlantic salmon, Salmo salar L.Journal of Fish Diseases, 1986
- Sulfonyl Fluorides as Inhibitors of Esterases. II. Formation and Reactions of Phenylmethanesulfonyl α-Chymotrypsin*Biochemistry, 1964