The Effect ofBrassica oleraceaStigma Extracts on the Germination ofB. oleraceaPollen in a Thin Layer Chromatographic Bioassay

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Abstract
Hodgkin, T. and Lyon, G. D 1986. The effect of Brassica oleracea stigma extracts on the germination of B. oleracea pollen in a thin layer chromatographic bioassay.—J. exp. Bot. 37: 406–411. A procedure for germinating Brassica oleracea pollen on thin layer chromatography plates pretreated with 20 mol m−3 tris(hydroxymethyl) methyl-aminopropanesulphonic acid (TAPS) buffer, pH 8·0 has been devised and used to detect pollen germination inhibitors in B. oleracea stigma extracts. Inhibitory zones in extracts of stigmas, unpollinated, or collected 0·5, 4, 8 and 24 h after self- or cross-pollination, differed little in RF values and sizes. Extracts of stigmas collected 1 h and 2 h after self-pollination gave a small additional inhibitory zone which was not detected in 1 h and 2 h cross-pollinated stigma extracts. The results showed some differences from those obtained using Petunia hybrida pollen germinated on T.L.C. plates that were not pretreated with buffer. The nature of the differences between the two bioassays is discussed and some possible reasons for them indicated.