Isolation of protein bodies on sucrose gradients
- 1 January 1972
- journal article
- Published by Springer Nature in Planta
- Vol. 104 (3) , 185-194
- https://doi.org/10.1007/bf00387073
Abstract
Storage protein bodies from sunflower cotyledons during early stages of seed germination were isolated on sucrose density gradients by isopycnic centrifugation. The density of this organelle on the gradients ranged between 1.26 and 1.36 g cm-3. A proteinase with a pH optimum of 5.2 was associated with this organelle, and is probably responsible for degradation of storage protein. A NADH-dependent cytochrome-c reductase, a membrane marker enzyme with a pH optimum of 8.4, was also present in this organelle fraction.Keywords
This publication has 26 references indexed in Scilit:
- Development of Microbodies in Sunflower Cotyledons and Castor Bean Endosperm during GerminationPlant Physiology, 1971
- 3-Phosphoglycerate Phosphatase in PlantsPlant Physiology, 1971
- Characterization of the fine structure and proteins from barley protein bodiesPhytochemistry, 1971
- Protein Bodies of Germinating Seeds ofVicia faba: CHANGES IN FINE STRUCTURE AND BIOCHEMISTRYJournal of Experimental Botany, 1970
- Enzymes Associated with Protein Bodies Isolated from Ungerminated Barley SeedsPlant Physiology, 1969
- Gibberellic Acid-Induced Synthesis of Protease by Isolated Aleurone Layers of BarleyPlant Physiology, 1967
- Influence of the Embryonic Axis on Protein Hydrolysis in Cotyledons of Cucurbita maximaPhysiologia Plantarum, 1967
- Proteolytic Enzymes and Endogenous Trypsin Inhibitor in Germinating Lettuce SeedsPhysiologia Plantarum, 1965
- Intracellular Distribution of Proteins in Pea CotyledonsPlant Physiology, 1963
- Purification and some properties of a protease from pea seeds, Pisum sativum L, s.sp. arvense A. and G.Biochimica et Biophysica Acta, 1960