Production and Purification of the Gamma Haemolysin of Staphylococcus aureus 'Smith 5R'
- 1 January 1976
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 92 (1) , 1-10
- https://doi.org/10.1099/00221287-92-1-1
Abstract
The .gamma. hemolysin of S. aureus Smith 5R was produced on Dolman-Wilson agar overlain with cellophane. Maximal yields of crude lysin with titers of 2000-4000 hemolytic units/ml were obtained in under 24 h at 37.degree. C in 10% (vol/vol) CO2 in air, on medium adjusted to pH 7.0. The crude lysin was purified 2700-fold (with 75% recovery) by ultrafiltration, gel filtration and (NH4)2SO4 fractionation. The specific activity of the lysin was 105 hemolytic units/mg protein after the dialyzed active precipitate was extracted with NaCl and reprecipitated with (NH4)2SO4. Purified .gamma. lysin was homogeneous by disc electrophoresis and immunoelectrophoresis.This publication has 6 references indexed in Scilit:
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