An enzyme rate equation for the overall rate of reaction of gel‐immobilized glucose oxidase particles under buffered conditions. I. Pseudo‐one substrate conditions

Publisher Website
Google Scholar
Abstract
The overall rate of reaction of buffered gel‐immobilized glucose oxidase particles is described by means of an enzyme rate equation which relates the overall reaction rate of a particle to the free solution characteristics of the enzyme, the effective diffusivity of the limiting substrate in the gel, the characteristic particle size, and the limiting substrate concentration adjacent to the gel surface. This equation accounts quantitatively for the limitation of the overall rate of reaction by substrate diffusion, and it is used to illustrate the influence of the system parameters, i. e., particle size, enzyme concentration, and pH, on the extent of the diffusional resistance associated with gel‐immobilized glucose oxidase particles.The enzyme rate equation is generally applicable to those enzymes whose kinetics approximately follow Michaelis‐Menten form when in free solution.

This publication has 17 references indexed in Scilit: