Isotype-Specific Suppression of IgE Synthesis in a Human Myeloma Cell Line (U-266) by an IgE-Binding Protein Derived from Normal Human Peripheral Blood Cells

1 January 1989

journal article
Published by S. Karger AG in International Archives of Allergy and Immunology

Vol. 88 (1-2) , 114-115
https://doi.org/10.1159/000234759

Abstract

Human peripheral blood cells produce immunoglobulin-binding factors upon stimulation with purified protein derivative. The binding factors from 2 healthy individuals were purified by IgE affinity chromatography. IgE-suppressive activity was demonstrable using the IgE-producing human myeloma cell line U-266 as an assay system. The effect of the suppressive factor seems to be reversible, isotype specific, as the immunoglobulin synthesis in the IgGk-producing U-1958 myeloma line was not affected, and selective for the epsilon heavy chain. The results also suggest that this suppression is not the result of a decrase in DNA synthesis or suppression of protein synthesis in general.

Keywords

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