D genome donors for Aegilops crassa (DDMcrMcr, DDD2D2McrMcr) and Ae. vavilovii (DDMcrMcrSpSp) deduced from esterase analysis by isoelectric focusing.

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Abstract
Putative D genome donors to A. crassa (2n = 4x = 28, DDMcrMcr and 2n = 6x = 42. DDD2D2McrMcr) and A. vavilovii (2n = 6x = 42, DDMcrMcrSpSp) were studied for esterase isozyme similarities. Esterase isozymes examines by gel isoelectrofocusing reveal no inter- or intraspecific variation in A. crassa and A. vavilovii. The putative parents, A. squarrosa (2n = 2x = 14, DD) and A. comosa (2n = 2x = 14, MM), each exhibited 3 isozyme phenotypes. The natural tetraploid form of A. crassa has an isoenzyme pattern corresponding to a mixture of esterases from type 2 squarrosa and type 1 comosa. The D genome donor to A. crassa 4x is type 2 squarrosa. The hexaploid form of A. crassa possesses the same D genome as the tetraploid form, suggesting a duplication of the type 2 squarrosa D genome. The D genome of the hexaploid form is the same as the D genome of Triticum aestivum cv. Chinese Spring (bread wheat, 2n = 6x = 42, AABBDD). The putative D genome donor to A. vavilovii corresponds to type 2 squarrosa.