The dexamethasone receptor in the Novikoff Hepatoma. Characterization and changes in concentration and nuclear uptake during glucocorticoid therapy
- 1 December 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 152 (3) , 689-695
- https://doi.org/10.1042/bj1520689
Abstract
Novikoff hepatoma ascites cells, grown intraperitoneally in rats, are shown to possess a high-affinity, low-capacity dexamethasone-binding protein. The receptor protein has an intracellular localization and concentration, association constant (Ka), glucocorticoid specificity and nuclear uptake in the presence of dexamethasone comparable with those of the G-protein of rat liver. During therapy of the tumour-bearing animal with cortisol, marked cyclic variations were observed in the concentration and nuclear uptake of the putative G-protein in the tumor cells; more transient variations were also observed in the Ka value of the receptor protein.Keywords
This publication has 19 references indexed in Scilit:
- 5-azacytidine-modified patterns of uridine kinase activities in normal and neoplastic tissues.1974
- Glucocorticoid-receptor complexes in rat thymus cells. "Cytoplasmic"-nuclear transformations.1974
- Suppression of DNA Synthesis in Hepatoma Cells Exposed to Glucocorticoid Hormone In VitroProceedings of the National Academy of Sciences, 1973
- Nature of Nuclear Acceptor Sites for Glucocorticoid- and Estrogen-Receptor ComplexesJournal of Biological Chemistry, 1973
- Interaction of glucocorticoids with rat liver nuclei. I. Role of the cytosol proteinsBiochemistry, 1973
- Specific cytoplasmic glucocorticoid hormone receptors in lactating mammary glandsBiochemistry, 1973
- Glucocorticoid-Binding Proteins in Human Acute Lymphoblastic Leukemic Blast CellsJournal of Clinical Investigation, 1973
- The principal glucocorticoid binding macromolecule in hepatoma cells in culture is similar to corticosteroid Binder II of rat liver cytosolBiochemical and Biophysical Research Communications, 1973
- Filter assay for steroid hormone receptorsBiochemistry, 1973
- The Relationship between Glucocorticoid Binding and Tyrosine Aminotransferase Induction in Hepatoma Tissue Culture CellsProceedings of the National Academy of Sciences, 1970