Cloning of the Escherichia coli K-12 hemB gene
Open Access
- 1 February 1988
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 170 (2) , 1021-1025
- https://doi.org/10.1128/jb.170.2.1021-1025.1988
Abstract
An Escherichia coli heme-requiring, heme-permeable mutant had no detectable 5-aminolevulinate dehydratase or porphobilinogen deaminase activities. The gene which complemented this mutation was cloned to a high-copy-number plasmid, and porphobilinogen deaminase activity was restored to normal levels, but the synthesis of 5-aminolevulinate dehydratase increased 20- to 30-fold. A maxicell procedure confirmed that the gene cloned was hemB.This publication has 50 references indexed in Scilit:
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