Honokiol Induces Apoptosis via Cytochrome c Release and Caspase Activation in Activated Rat Hepatic Stellate Cellsin vitro
- 27 January 2005
- journal article
- letter
- Published by Georg Thieme Verlag KG in Planta Medica
- Vol. 71 (1) , 82-84
- https://doi.org/10.1055/s-2005-837757
Abstract
The therapeutic goal in liver fibrosis is the reversal of fibrosis and the selective clearance of activated hepatic stellate cells (HSCs) by inducing apoptosis. Over the past several years, we have screened for natural products that mediate apoptosis in activated HSCs. Among the candidate compounds, honokiol, isolated from Magnoliae cortex, was found to induce apoptotic death in activated rat HSCs, while there was no cell viability change in hepatocytes, at concentrations of 12.5 - 50 μM. Apoptosis was identified by DNA fragmentation, activation of caspase-3 and -9, and the proteolytic cleavage of poly(ADP-ribose) polymerase, down-regulation of bcl-2 and the release of mitochondrial cytochrome c into the cytoplasm. HSCs:hepatic stellate cells MTT:3-(4,5-dimethylthiazol-1-yl)2,5-diphenyltetrazolium bromide PARP:poly(ADP-ribose) polymeraseKeywords
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