Untersuchungen über Sauton-Tuberkulin und seine Reinigung

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Abstract
100 ml. of Sauton tuberculin of 8 weeks growth were treated with 1 ml. glacial acetic acid, and the precipitate formed removed by centrifugalization. To the clear soln., 15 ml. of 20% tannin soln. were added, and the moist tannin precipitate treated with 0.2 ml. 5% NH3, and reprecipitated with 20 ml. acetone containing 0.06 ml. conc. HCl. After repeated reprecipitation, washing and drying, 50 mg. of ''a highly active tuberculin in powder form were obtained. A still more active tuberculin was prepd. by electrodialysis of purified tuberculin by the use of cuprophane membrane. Its lethal dose was 0.07 mg. and the skin-reaction dose, 0.00008 mg. In cultures of the human tuberculosis bacillus, the amt. of protein substances precipitated with tannin reached a maximum in 60 days, while the amt. of substances precipitated with the acidified acetone alone continued to increase with time. The later increase was due to a continued increase in carbohydrate bodies formed by the bacillus. The specific activity of tuberculin was proportional to the amt. of protein substances precipitated with tannin.

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