Regional localization of aβ-galactosidase locus on human chromosome 22
- 1 July 1977
- journal article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 3 (4) , 351-363
- https://doi.org/10.1007/bf01542965
Abstract
Human white blood cells with an X/22 translocation [46, XX, t(X; 22)(q23; q13)] were fused with Chinese hamster cells. The isolated hybrids were analyzed for human chromosomes and 21 enzyme markers. An electrophoretic technique for studying the β-galactosidase isoenzymes in man-Chinese hamster hybrid cells was developed. Immunological studies showed that the β-galactosidase marker studied in these hybrids did contain immunological determinants of human origin. Furthermore the results provided evidence that a locus for β-galactosidase is situated on chromosome 22 distal to the breakpoint in q13.Keywords
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